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文章摘要
魏凌霄,刁晓平,张钦洲.氨磺乐灵对蚯蚓抗氧化酶活性及基因表达的影响[J].海南大学学报编辑部:自然科学版,2019,37(2):.
氨磺乐灵对蚯蚓抗氧化酶活性及基因表达的影响
Effects of Oryzalin Stress on Antioxidant System Enzyme Activity and Gene Expression of Earthworms
投稿时间:2019-02-25  修订日期:2019-03-19
DOI:10.15886/j.cnki.hdxbzkb.2019.0022
中文关键词: 氨磺乐灵; 赤子爱胜蚓; 酶活性; 基因表达
英文关键词: oryzalin,Eisenia foetida,antioxidant enzymes activity,gene expression
基金项目:中央高校基本科研专项资金(FRF-BD-17-016A)
作者单位E-mail
魏凌霄 1 海南大学热带农林学院 2 海南大学南海海洋资源利用国家重点实验室 weilx123@foxmail.com 
刁晓平 海南大学南海海洋资源利用国家重点实验室 diaoxip@hainu.edu.cn 
张钦洲 1 海南大学热带农林学院 2 海南大学南海海洋资源利用国家重点实验室  
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中文摘要:
      以赤子爱胜蚓(Eisenia foetida)为实验对象,研究了不同浓度(0、2.5、5、10、15 mg·kg-1)的氨磺乐灵对蚯蚓体内抗氧化酶(SOD、CAT、GSH ST)活性和相应基因表达的影响结果表明:暴露21天后,蚯蚓体内的SOD、CAT、GSH ST活性均随着氨磺乐灵浓度的增高而降低,其中,在2.5 mg·kg-1组中,三种酶活均显著高于其他组;三种酶的基因表达均在低氨磺乐灵的组上调,而在高氨磺乐灵的组下调,三种酶的基因表达在5 mg·kg-1组明显上调(p<0.01),CAT和GST在高浓度组(15 mg·kg-1)的表达显著下调(p<0.01和p<0.05)研究表明:蚯蚓体内的SOD、CAT和GSH ST活性和相应基因表达对氨磺乐灵的土壤污染响应敏感,可以将其作为监测氨磺乐灵土壤生态毒性效应的生物标志物。
英文摘要:
      Eisenia foetida was used to study the effects of oryzalin at different concentrations (0, 2.5, 5, 10, 15 mg?kg-1) on activities of total superoxide dismutase (T-SOD) ,catalase ( CAT) ,glutathions S-transferase(GST) and the relative expressions of SOD,CAT and GST gene in earthworm. The results showed that the activities of SOD, CAT and GST were first activated and then decreased with the increase of oryzalin concentration after 21 days of exposure. The activities of this three enzymes in Eisenia foetida by exposure to oryzalin at 2.5 mg?kg-1 soil were significantly higher than those at the other dose level. The relative expression levels of the three enzymes were up-regulated in the low-concentration group and down-regulated in the high-concentration group. All antioxidant enzyme gene relative expressions were significantly up-regulated in Eisenia foetida by exposure to oryzalin at 5 mg?kg-1 soil(p<0.01).CAT and GST gene expression were significantly down-regulated in Eisenia foetida by exposure to oryzalin at 15 mg?kg-1 soil(p<0.01 and p<0.05).All these results showed that the three antioxidant enzyme activities and their relative expression of genes were sensitive to the soil under the stress of oryzalin, which could be used as potential biomarkers to monitor the ecotoxic effect of oryzalin on earthworms.
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