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文章摘要
张维炜,梁业松,何靖,李岩强,陈帅龙,张浩,叶恒振,王茜,骆剑.雌核发育棕点石斑鱼EST-SSR的开发验证[J].海南大学学报编辑部:自然科学版,2019,37(2):.
雌核发育棕点石斑鱼EST-SSR的开发验证
Development and Verification of EST-SSRs Based on Transcriptome of the Gynogenetic Epinephelus fuscogutatus
投稿时间:2019-01-10  修订日期:2019-03-12
DOI:10.15886/j.cnki.hdxbzkb.2019.0021
中文关键词: 雌核发育; 棕点石斑鱼;转录组微卫星标记;遗传多样性
英文关键词: Gynogenesis, Epinephelus fuscogutatus, EST-SSR, Genetic diversity
基金项目:海南省重点研发计划课题(ZDYF2016085,ZDYF2017038);国家“863”计划项目(2012AA10407)资助
作者单位E-mail
张维炜 南海海洋资源利用国家重点实验室 1149057128@qq.com 
梁业松 南海海洋资源利用国家重点实验室  
何靖 南海海洋资源利用国家重点实验室  
李岩强 南海海洋资源利用国家重点实验室  
陈帅龙 南海海洋资源利用国家重点实验室  
张浩 南海海洋资源利用国家重点实验室  
叶恒振 南海海洋资源利用国家重点实验室  
王茜 南海海洋资源利用国家重点实验室 wangqian170@163.com 
骆剑 南海海洋资源利用国家重点实验室  
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中文摘要:
      通过人工雌核发育棕点石斑鱼(Epinephelus fuscogutatus)的转录组序列,共获得了24 359个微卫星位点,棕点石斑鱼转录组的微卫星重复基序具有不同的分布特征,其中以单碱基(34.05 %)、二碱基(37.58 %)和三碱基(22.75 %)为主要的 EST SSR 基序重复类型根据微卫星引物设计原则,随机筛选了93个 EST SSR 位点来进行引物合成和多态性检测,最终开发了48个具有多态性的微卫星标记,并在不同棕点石斑鱼群体中进行了初步的验证结果显示:每个位点等位基因数(Na)为2~22, 平均等位基因数为9;观测杂合度(H0)为0.111~1.000,期望杂合度(He)为0.636~0.940;多态信息含量(PIC)为0.538~0.922个,表明48个 EST SSR 位点均表现出高多态性 ( PIC > 0.5)结果表明:基于雌核发育棕点石斑鱼转录组数据,开发微卫星标记是可行的本研究所开发的具有多态性的微卫星标记可应用于棕点石斑鱼及其近缘种的遗传多样性分析和分子标记辅助育种研究。
英文摘要:
      A total of 24,359 microsatellite loci were obtained from artificial gynogenetic Epinephelus fuscogutatus transcriptome. Mononucleotide, dinucleotide and trinucleotide repeats were the main types, respectively for 35.05%, 37.58%, 22.75% in the total SSR. According to the design principles of microsatellite primers, 93 EST-SSR loci were randomly selected for primer synthesis and polymorphism detection. Finally, 48 polymorphic microsatellite markers were developed and used in different E. fuscogutatus groups. The results showed that the number of alleles (Na) was ranged from 2 to 22, with an average of 9 alleles /locus. The observed heterozygosity (Ho) was ranged from 0.111 to 1.000, and the expected heterozygosity (He) was ranged from 0.636 to 0.940. The polymorphism information content (PIC) ranged from 0.538~0.922, indicating that 48 EST-SSR loci showed high polymorphism (PIC > 0.5).?This study indicated that EST sequences produced by transcriptome sequencing was an effective source to develop SSR markers. It was feasible to develop microsatellite markers based on transcriptome data of the gynogenetic E. fuscogutatus, and the developed microsatellite markers with polymorphism could be applied to genetic diversity analysis and molecular marker-assisted breeding research of E.?fuscogutatus and sibling species.
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