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文章摘要
周莹,韩玉龙,骆剑,王茜,陈国华.鞍带石斑鱼、棕点石斑鱼及其杂交子代DNA甲基化MSAP分析[J].海南大学学报编辑部:自然科学版,2017,35(2):.
鞍带石斑鱼、棕点石斑鱼及其杂交子代DNA甲基化MSAP分析
Analysis of Genomic DNA Methylation on Epinephelus lanceolatus, Epinephelus fuscoguttatus and their Hybrid Generation by Methylation-Sensitive Amplification Polymorphism.
投稿时间: 2017-02-26  最后修改时间: 2017-04-17
DOI:
中文关键词: 石斑鱼;DNA甲基化;甲基化敏感扩增多态性;杂种优势
英文关键词: Grouper; DNA methylation; MSAP; Heterosis
基金项目:国家863计划项目“重要南方海水养殖鱼类良种培育”(2012AA10A407),海南省重点研发项目“石斑鱼人工雌核发育良种培育体系的建立”(ZDYF2016085)资助。
作者单位E-mail
周莹 南海海洋资源利用国家重点实验室 1499364556@qq.com 
韩玉龙 南海海洋资源利用国家重点实验室  
骆剑 南海海洋资源利用国家重点实验室 luojianfish@aliyun.com 
王茜 南海海洋资源利用国家重点实验室  
陈国华 南海海洋资源利用国家重点实验室  
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中文摘要:
      DNA甲基化是真核生物重要的表观遗传学修饰,甲基化程度的改变被证实是杂种优势产生的主要机制之一。为了探究海洋鱼类的杂交优势产生机制,本文以鞍带石斑鱼、棕点石斑鱼及其杂交子一代石斑鱼为对象,采用甲基化敏感扩增多态性(MSAP)技术对三个群体的基因组DNA胞嘧啶甲基化修饰水平进行研究。实验结果显示,棕点石斑鱼、鞍带石斑鱼、杂交子一代石斑鱼的基因组DNA总甲基化率分别为57.18%、63.16%、54.76%,DNA甲基化程度在石斑鱼亲本基因组中较高,而在杂交子代中降低。三个群体的DNA全甲基化率分别为31.66%、39.71%、40.00%,半甲基化率分别为25.52%、23.44%、14.76%,杂交子代的半甲基化率显著低于亲本。研究表明,鞍带石斑鱼与棕点石斑鱼的杂交子代在基因组层面上和双亲相比发生了较大的甲基化水平的调整,不同位点DNA甲基化的增强或减弱对石斑鱼杂种优势可能产生影响。
英文摘要:
      As one of the most common epigenetic modification in eukaryotic cell, DNA methylation should be the important foundation of heterosis in plants and animals. To investigate the mechanism of heterosis in fish hybrids, the differences in DNA cytosine methylation between Epinephelus fuscoguttatus, Epinephelus lanceolatus and their hybrids populations had been studied via methylation-sensitive amplification polymorphism (MSAP). The results showed that MSAP values of the three groups were respectively 57.18%, 63.16% and 54.76%. The full-methylation ratios of these populations were 31.66%,39.71% and 40.00%, and the half-methylation ratios were 25.52%,23.44% and 14.76%.The DNA methylation level of the hybrids were in general lower than the parental lines, in addition, the hybrids had more abundant methylation patterns. The results confirmed that the groupers had significantly adjusted DNA methylation patterns in genomic DNA level after hybrid. It also showed the heterosis of the groupers should benefit from the DNA methylation and DNA demethylation.
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